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KMID : 0545120110210080846
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 8 p.846 ~ p.853
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Production of 1,2-Propanediol from Glycerol in Saccharomyces cerevisiae
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Jung Joon-Young
Yun Hyun-Shik
Lee Jin-Won
Oh Min-Kyu
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Abstract
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Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2- propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.
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KEYWORD
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Saccharomyces cerevisiae, glycerol, 1, 2-propanediol, metabolic engineering
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